FEEDING PATTERN AND SPOROZOITE INFECTION IN ANOPHELINES AND BIOLOGICAL CONTROL OF MOSQUITOES

Abstract

Anopheline surveys were conducted during June 1987 t o May 1988 in Islamabad-Rawalpindi and its vicinities and fif teen different a nopheline species were col l ect ed from the area under study . For bloodmeal i dentification e nzyme- linked i mmunosorbent assay was used. 9.78 percent Anopheles culicifacies and 13.65 percent An . fluviati is were found as positive t o human blood. Sporozoite infections wer e detected in Anopheles fluviatilis that was ear l ier cosnidered as suspe cted malaria vector . Becillus thuringiensi~ H-14, Bacillus sphaerlcus 1593, Melia azadirachta L and Me l ia azedarach L. were tried against Pakistan Mosquito specLes. Baci llus thuringiensis H-14 was found as good f or surface fee ding anophelines and Bacillus sphaericus for bottom feedOng culicines . Melia azadirachta Land Melia azedarach L extract in methanol was found as highly toxic to mosquito larvae . Considering the aquatic f eeding h abits domestic ducks and nine di f ferent fish were tried f or mosquito control and i t was observed t h a t domestic ducks and chidu fish are suitable for mosquito contro l . Extracts from both, Neem and Bakain were also used i n combinat i on with Chidu fish and Bacillus thuringiensis H-14 and good larval contro l was ach ieved.