Synthesis and biological evaluation of metallic complexes of crude extracts of Bergenia ciliata and Euphorbia wallichii

Abstract

Secondary metabolite obtained from plants play important role in treatment of various diseases, both in natural or coordinated forms. Metals combined with such compounds play key role in treatment strategies of different ailments. Current study deals with biological evaluation of fourteen metallic complexes, synthesized using extracts of Bergenia ciliata (BC) and Euphorbia wallichii (EW). Complexes showed increased reducing potential whereas decreased activity was observed in DPPH scavenging assay and total antioxidant assay. Cytotoxic potential of complexes was evaluated by performing brine shrimp assay. EWHg (LD50= 96 μg/ml) and BCFs (LD50=58 μg/ml) complexes showed higher cytotoxic potential comparative to their extracts. Two gram positive bacterial strains i.e. M. luteus ATCC 10240, S. aureus ATCC 6538 and four gram negative strains i.e. A. aerogens ATCC 13048, E. coli ATCC 15224, S. typhimurium ATCC 14028 and B. bronchiseptica ATCC 4617 were used for evaluation of antibacterial activity of complexes. EWHg complex was active against five bacterial strains and exhibited maximum inhibition zone (16±1.5 mm) among EW complexes. BCHg showed highest activity among BC complexes and produced inhibition zone of 17±0.58 mm against M.luteus. Five fungal strains, A. fumigatus (FCBP 66), A. niger (FCBP 0198), F. solani (FCBP 0291), A. flavus (FCBP 0064), and Mucor species (FCBP 0300) were used in present study to evaluate the antifungal potential of complexes. EWFs produced maximum zone (10±0.2 mm) against A. flavus. Five BC complexes were found to be active against A. niger and A. fumigatus. BCAl produced maximum zone of inhibition (16±2.0 mm) against A. niger. All complexes were evaluated for protein kinase inhibition potential in which, EWCd, EWCs, BCFs and BCFc complexes produced zones of inhibition ranging from 9 mm to 16 mm. FTIR of extracts and complexes was also performed which suggested the presences of various phytochemicals such as hydrocarbons, terpenoids, abietane steroids, aromaticeasters, phenolics, flavone glycosides and several bioactive constituents.