Evaluation of Protective Effects of Vitamin C against Toxicity Induced by Furan in Adult Male Sprague Dawley rats

Abstract

Furan is a heterocyclic organic molecule. Furan causes adverse effects on the vital organ of rodents such as the liver, kidney, pancreatic, and renal cells. In past studies, it is also reported that furan causes toxicity in the endocrine and reproductive system in male rats. The current study was designed to examine the protective effect of Vit C against toxicity induced by furan in male Sprague Dawley rats. The experiment was conducted for 28 days. Rats were randomly divided into four groups (n = 5). Group-I was control, received corn oil, and while Group II was treated with Furan 30 mg/kg orally, Group III was given 200 mg/kg orally, Group IV was given Vit C 200 mg/kg, and furan 30 mg/kg. Vitamin C significantly decrease the TBARS and ROS levels in all tissues exposed to furan as compared to the control group While POD, CAT and SOD were (P<0.05) significantly lowered in the furan treated group. The furan treatment group showed a significant reduction in testosterone concentration as compared to the to ist group. Furan-treated groups showed alterations in the normal morphological architecture of testis. Furan caused a reduction in the number of spermatids and disruption of tubules. A non-significant decrease in the organ mass index was also observed in furan treated group. Vitamin C was found to be an antioxidant role that reduced furan-induced toxicity. Vitamin c reduced alterations in oxidative stress, as it also restored the morphological alterations as compared to the furan treatment group. Vitamin C also restored the level of testosterone. It is concluded vitamin C is beneficial to furan-induced toxicity in male Sprague Dawley rat.