Computational Design of a Vaccine for Helicobacter Pylori

Abstract

To control the drug abuse and diseases caused by the Illultidrug-resistant pathogen helicobaetcr pylori, a preventative strategy has applied which is the development of potent vaccine. H. pylori is a highly infectious bacteria that makc colonies in the gastric mueOS'1 of about 50% of the total population. The International Agency for Research on Cancer (I ARe) assessed that gastric cancer is the yd prominent reason of death in the World. World Health Organization (W HO) classified H. pylori as a type I carcinogen. Thc pathogen infections arc significantly of greater risk to public health in countries of low socioeconomic status. N. pylori leads to gastrointestinal tract dise<1ses starting from gastric ulcers 10 gastric atrophy and uhimately ending up with gastric adenocarcinoma, gastric cancer and mucosa-associated lymphoid tissue (MALT) lymphoma. The pathogen proficiently eludes inherent immune detection of the host and obstinately try to make colonies in the host body. H. pylori uses specific virulcnt factors (VFs) to cause pathogenesis. Among them, thc most important is cytotoxin associated gene pathogenicity island (cagPAI) and vacuolating cytotoxin gcnc A (VacA). The infection caused by this pathogen is typic<1lly attained during early childhood and it tcnds to endure in the body unless it is treated. Because the antibiotics used in the anli-f/. pylori therapy arc increasingly showing resistancc, so the necd to develop a polent vaccine is still remains CI challenge. Thus, using the most efficient bioinfonnatics tools, an effcctive, proficient and a rational vaccine design against the pathogen H. pylori is conceivable. In this study, multiple computational approaches wcre used to design a llluitiepitope subunit vaccine COllstl1lct ngainst Helicobac(lter pylori to induce bot" (l(laptive ami innate immullity. Based 011 i/llllllllwilyorlllatics alld subtractive proteomics oj H. pylori proteome, we "ave prioritized fOllr targel protei"s: inner membrane flagellar protein niL, putative outer membrane prolein A (1-1 P _09 I 2), extracellular fJagellin B protein flaB and extracellular flagellar basal body rod protein flgG wliich are satisfying tile slandards of vaccine COlltenlion. Our findings suggest that our Illultiepitope vaccine construct against H.pylori strains could serve as a promising anti-f1.pylori vaccine candidate.