Biological Evaluation of Nanomedicine in Combination with Chemotherapy

Abstract

Over the last few decades, advancement in the field of nanomedicine revolutionized the pharmaceutical industry worldwide. Due to innovation of nanomedicines, patient health improved greatly leading to development of novel therapeutic products using nanomedicines. Biochemical properties and biocompatibility of these nanomedicines make them suitable for their use against various diseases. In this study, we examine the biochemical properties of caffeine-coated Mesoporous silica-based Nanoparticles (MSNPs), a nanomedicine and their combination with chemotherapy via in vivo and in vitro assays. XRD, FTIR. UV visible spectroscopy confirmed caffeine loading on MSNPs. The cytotoxic potential of nanomedicines and its combination in a living cell was inspected by performing a Brine shrimp assay. Total reducing power assay and total antioxidant assay were performed to evaluate antioxidant potential. DPPH assay was done to determine the free radical scavenging activity of nanomedicine and its combination. The Musculus mouse model determines the antidepressant, analgesic, anticoagulant potential of caffeine-coated MSNPs and doxorubicin. Comparing with the control result of these assay shows that nanomedicine exhibit excellent cytotoxic and antioxidant potential at 2mg/ml concentration P<0.05.By comparing in vivo assay results the analgesic, anticoagulant and antidepressant activity of nanomedicines also show promising efficacy. Therefore it concluded that nanomedicines and chemotherapy are promising therapeutic strategies against various diseases due to efficient drug delivery and unique biochemical properties.