Effective Potential of Stem bark of Dodonaea Viscosa against CCl4 induced Hepatotoxicity in Rat

Abstract

Incidence of inflammation and fibrocystic disease in hepatocytes upon treatment with CCl4 is a widely accepted phenomenon to determine sanative ability of testing subject. The leaves, flowers and aerial parts of Dodonaea Viscosa are regionally used to treat skin diseases, diarrhea, snake bites, and stomach injuries. The current study aims to determine the protective propensity of D.viscosa stem bark methanol extract against CCl4 induced liver impairments. Exploratory qualitative and quantitative phytochemical testing was done to ascertain the inherence of phytochemicals in D.viscosa stem bark methanol extract. Before investigational testing on murine models, the forestalling ability of D.viscosa in response to redox damage and inflammation was determined through in-vitro antioxidant assays; DPPH assay, FRAP assay, PM assay, and Ferrous ion chelating assay and heat-induced protein denaturation inhibition assay respectively. The in-vitro assays exhibited promising results in terms of IC50 values depicting the profound restoring potential of the D.viscosa stem bark extract. In-vivo investigation was done on murine models at biochemical, anatomical and molecular levels. Optimistic results were obtained in biochemical analysis; the levels of serum markers and hepatic enzymes were effectively re-established by plant treatment, comparable to normal group. Similar trend of outcomes was observed in histological and molecular analysis where plant treatment constructively amended the dire repercussions of CCl4 and restored cellular anatomy and mRNA levels of ER stress, inflammatory, fibrotic and apoptotic genes, thus designating the potential efficacy of DVME in opposition to hepatic toxicity in rat. Our findings corroborate that the methanol extract of stem bark of Dodonaea visocsa has potential to quench the reactive radicals thus aids in relieving hepatic inflammation