A biochemical approach to evaluate the toxic effect of Pyriproxyfen on reproductive system of adult male Sprague Dawley rats

Abstract

Pyriproxyfen is a fenoxycarb derivative a juvenile hormone analog. Pyriproxyfen causes unpropitious effects on vital organ of rodents such as kidney, pancreases and liver. In previous studies, it is noted that pyriproxyfen causes endocrine and reproductive toxicity. The objective of this study is to calculate harmful consequences induced by pyriproxyfen in male Sprague Dawley rats. The experiment was conducted for 28 days. Rats were distributed into four groups (n=5). Group-I considered as control, received normal saline. Group –II treated with pyriproxyfen 64mg/kg, Group-III was given 124mg/kg, and Group-IV was given 186mg/kg orally. All the animals were weighed and sacrificed by decapitation on day 29. Plasma was extracted from blood samples and kept at -20°C for biochemical examination. Pyriproxyfen non-significantly decrease the body and accessory organ weights. While the weight of testis decreases significantly. Pyriproxyfen administration induce a notable (p<0.001) decrease in DSP. in treated groups remarkable reduction in blood glucose concentration (p<0.001) was detected. The results showed that biochemical markers serum total protein content, cholesterol, LDH, Triglycerides increased significantly. Pyriproxyfen-treated groups displayed a significantly lower testosterone concentration as compared to control group. Plasma cortisol level increased significantly in high dose treated groups. The study concluded that pyriproxyfen decreases body and accessory organ weight, increases oxidative stress and reproductive dysfunctions in testis