Evaluation of oral subchronic administration of Pyriproxyfen on steroidogenic activity of ovaries in adult Sprague Dawley rats.

Abstract

Pesticide exposure such as PPF in a variety of animals is known to possess an endocrine disruptive potential with effects on multiple aspects of reproduction. PPF is a pyridine ring-containing heterocyclic molecule derived from natural sources and categorized to the group of biopesticides. The aim of present study is to evaluate the toxic effects of Pyriproxyfen on reproductive system of adult rats by determining ovarian function. For this purpose, twenty adult female Sprague Dawley rats (n=5/group) weighted (160±15g) were taken. Control received distilled water, while Group I, II, and III received 62mg/kg, 124mg/kg and 186mg/kg of Pyriproxyfen for 28 days. On day 29, animals were weighed, decapitated, and blood was collected, centrifuged and plasma was stored at -20˚C until analysed. Reproductive organs, including, ovaries and uterus were dissected and weighted. The length was measured using measuring tape and the subsequent formula was used to determine the BMI. Determination of blood glucose and estrous cyclicity was carried out with 2 weeks gap. The blood plasma levels for cortisol, progesterone and estradiol were also determined using multiple ELISA. The results showed a decrease in ovarian weight and BMI as compared to control while a remarkable increase in weights of reproductive organs and body organs was noticed. The significant increase (P<0.001) in blood glucose levels of G2 and G3 from day 1 to day 14 while a significant decrease (P<0.001) from day 14 to day 28 was observed. Alterations in estrous cycle was noticed with prolonged diestrus phase and shortened proestrus phase. The hormonal analysis depicted a reduction in cortisol, estradiol and progesterone concentration in PPF treated groups as compared to control. The study concluded that PPF decreases body weight, ovarian weight and BMI, increases the weight of other Evaluation of oral subchronic administration of Pyriproxyfen on steroidogenic activity of ovaries in adult Sprague Dawley rats. 1 Abstract reproductive and body organs such as heart, liver, pancreas, kidneys and brain, alterations in blood glucose levels and irregularity in the phases of estrous cycle, decreases concentrations of blood plasma cortisol, estradiol and progesterone. It can be suggested from the present findings that PPF exerts toxic effects on reproductive health of females and act as an endocrine disruptor, however, further similar studies can be designed to elucidated the underlying mechanisms responsible for inducing reproductive toxicity in animals, by monitoring actions of PPF on cellular interactions in ovaries and other target organs and their molecular pathways.