Micropropagation of Carnation, Lilium and Gladiolus on different Growth Hormone Supplemented Media

Abstract

The cut flower industry is an untapped market in Pakistan that relies heavily on importing flowers. Using tissue culture technology, it is possible to produce different types of flowers in large quantities that can be sold to local nurseries. Multiple varieties of carnations, lilium and gladiolus were used in the experiment to study the effects of different plant growth regulators such as auxins, cytokinin and gibberellins. Different concentrations of these hormones were added in MS media to determine their effects on the phenotypic traits of cut flowers. Using optimized sterilization techniques, it was observed that maximum growth for carnation was on C-1 medium (0.5 mg/L Kinetin + MS Medium) in terms of plant height and shoot induction. While C-3 medium (0.5 mg/L 1-Napthalene Acetic Acid + MS Medium) produced the highest formation of calluses. For lilium, LM-4 medium (4 mg/L Benzylaminopurine + 1 mg/L Indole-3 Acetic Acid + MS Medium) produced the best cultivars that had the highest number of multiple shoot inductions, the most plant height and the greatest number of leaves. In case of gladiolus, contamination was present in the intercellular spaces of tissues in all types of varieties, so it was not proceeded further.