Characterization of Gibberellic Acid Produced by Bacteria Isolated from Ghulkin Glacier, Hunza Valley

Abstract

The northern area of Pakistan has an ecosystem of cryogenic nature where plants are hard to grow and provide unfriendly environment for their sustainability. Commercial biofertilizers has evolved to be a tremendous remedy for such environment but they are not viable at such low temperature .The psychrophilic microbes of northern glaciers has the ability to grow in such environment and produce metabolites that are adopted to cold environment. gibberellic acid produced by organisms are good example of biofertilizers produced by natural source. The current study aims to screen, optimized., produce ,purify and characterized the cold adapted bacteria Ghul kin Glacier, Hunza Valley, Gilgit Baltistan, Pakistan. A total of 8 strains were screened for the production of gibberellic acid on nutrient broth production media. Fermentation conditions for the maximum production of gibberellic acid of all selected isolates were optimized for which productions were carried out in broth media using the optimized conditions. Purifications was performed by solvent-solvent extraction method. Characterization of the selected strains were carried out through, Fourier Transform Infrared Spectroscopy (FTIR) and Proton based Nuclear Magnetic Resonance (H-NMR). Two strains GA19 and GB16 shoes the production of gibberellic acid Maximum gibberellic Acid production by GB16 were recorded at15°C, pH 7, and peptone as carbon source after 96hours of incubation. The optimum conditions for the maximum production of gibberellic Acid by GA9 were recorded at 15°C, pH 7, AND PEPTONE as carbon source after 120 hours of incubation. The similarity of spectral peaks between standard gibberellic Acid and extracted gibberellic Acid from the selected isolates confirmed the production of hormone. Further confirmation was done by NMR by finding the functional groups present in gibberellic acid. These cold adapted strains isolated from the Ghulkin Glacier samples were good producers of gibberellic Acid which would be used as biofertilizers for crop growth promotion in the cold climatic regions of Pakistan.