Investigation of Bacterial Diversity in Cosmetics on the basis of 16S rRNA Sequencing

Abstract

Cosmetics products are one of the most essential and frequently used components in our daily life. Besides improving human health, they provide us healthy lifestyle and boost up our self esteem. Globally cosmetics market is projected from 287 billion USD in 2021 to 415 billion USD 2022. This research study aims at the isolation, identification and characterization of bacterial strains isolated from cosmetics. 14 bacterial colonies were isolated by inoculating different cosmetics products on Tryptic Soya Agar. All the strains showed optimum growth at 37°C. Different approaches were adopted for the identification of bacterial strains which include biochemical testing and molecular characterization. All strains were assessed through biochemical tests and were further proceeded for nucleotide sequencing. We identified six strains through Sanger sequencing. Phylogenetic analysis showed different bacterial strains were revealed in cosmetics products including Sphingomonas Paucimobilis, Cytobacillus oceanisediminis, Robertmurraya andreesnii, Cytobacillus Firmus, Falsibacillus pallidus, and Acinetobacter junii. Sphingomonas has potential of bioremediation and can be utilized for degrading toxic compounds to make the environment better. Similarly, Cytobacillus is found to be involved in bio mineralization and also aids in fermentation. Our results have shown that there is dire need to assure the strict safety regulations regarding cosmetics. Improper manufacturing practices can lead to the contamination of cosmetics which could lead to the severe consequences deteriorating the quality of health. Further studies are needed to explore the potential of these isolates so that they can be utilized to improve our health as well as environment.