Characterization of Anitimicrobial Cpmpounds Produced by Bacteria Isolated from Marine Soil.

Abstract

Antibiotics are the secondary metabolites of bacterial or fungus origin which inhibit the growth of other microorganisms. In the start it was been isolated from natural sources, due to decrease in the isolation from there it is now prepared synthetically. Several factors involve in the failure of antibiotic therapy. The failure is due to miss use of antibiotics that led to the evolution of multi-drug resistant pathogens like Methicillin resistant Staphylococcus aureus, which is important emerging pathogen. There is a large decline in the novel antibiotic production from several decades due to lower interest of pharmaceutical industries in the antibiotics. New strategies to compete with multi-drug resistant pathogens are in progress but there is still increase in demand for novel antibiotics. For this purpose new sites are being explored in the search for novel antibiotics. The objective of this study is to evaluate the antimicrobial potential of marine bacteria against MDR pathogens, optimize culture condition for production and produce antimicrobial from it. Initially the 172 marine isolate were screened from six soil samples for their antimicrobial production ability against A TCC bacteria and FFBP fungi. Then in secondary screening 84 isolates were screened for their activity against multi-drug resistant bacteria and fungi . One highly producer strain KSS 1.4 was selected from these 32 isolates which produce antimicrobial in secondary screening. The strain KSS 1.4 was biochemically characterized and different parameters were optimized for this isolate. The optimized temperature was 37 D C, starch was best carbon source, peptone as best nitrogen source, starch casein as best production medium, initial pH 5 as best pH, 50%:50% sea water and distilled water as best ratio, 2% NaCl presence in the media in case of distilled water were the optimized parameter for production of antimicrobial from isolate KSS 1.4. The optimized solvent for extraction was n-hexane. The results from this study reveal that the marine bacteria have a great potential to be use for antimicrobial production. The study from this environment will led to the discovery of new types of microorganisms and novel metabolites from them which will be of medical and industrial importance.