Diversity of Thermophilic Bacteria in Hot springs and Desert Soil of Pakistan and Identification of Some Novel Species of Bacteria

Abstract

A Gram negative strain labelled as NCCP-1258T, from Cholistan desert soil, Bahawalpur, Pakistan for which optimum growth conditions were detelmined for impoltant parameters like, media optimization, growth conditions for pH, temperature and salt concentration. Phylogenetic studies were perfOlmed with three algorithms based on 16srRNA gene sequence was aligned against sequences present in datab base of Ezbiocloud and comparison revealed that strain NCCP-1258T is in right place with genus Microvirga and its closest relative is Microvirga lotononidis. DNA-DNA similarity values ofNCCP-1258T was less than the 42% that is enough to delineate a new species. According to the criteria for describing a new species that belongs to Microvirga genus and other rhizobium species 16SrRNA gene sequence do not give accurate resolution and concatenated sequences of housekeeping gene (dnaK, gyrE, recA and rpoB) should also be amplified and compared for similarity. While following all criteria's strain NCCP-1258T was placed as a new species of genus Microvirga, for which the name Microvirga pakistanensis sp. nov. was proposed.